產(chǎn)品名稱(chēng):EE 肉湯 (緩沖葡萄糖煌綠膽鹽肉湯) EE Broth (Buffered Glucose Brilliant Green Bile Broth)
供應(yīng)商:Oxoid
貨號(hào):CM0317
規(guī)格:500g
產(chǎn)品說(shuō)明：
Directions
Add 43.5g to 1 litre of distilled water. Distribute 100ml quantities in 250ml flasks and heat at 100°C for 30 minutes only. Cool rapidly in cold running water. This medium is heat sensitive.
Technique
1. Resuscitate debilitated cells by incubating 1:10 dilutions of the food samples under investigation in Tryptone Soya Broth CM0129 at 25°C for 2-8 hours. The fluid layer should not be much deeper than one centimetre. Shake the flask to disperse the contents alternately in clockwise and anti-clockwise directions for 30 seconds on three successive occasions.
2. After the period of time necessary for resuscitation, ten-fold volumes of EE Broth are added to the resuscitated suspensions.
3. Shake to disperse as above. For large samples it is desirable to add the resuscitation medium containing the product under examination, to equal volumes of double strength EE Broth.
4. Incubate at 44°C for 18 hours for thermotrophic bacteria; 32°C for 24/48 hours for mesophilic bacteria; 4°C for 10 days for psychrotrophic bacteria depending on the groups of Enterobacteriaceae sought.
5. Examine the tubes of broth and look for turbidity with some change of colour towards yellowish-green for presumptive evidence of Enterobacteriaceae.
6. Subcultures can be made on to Violet Red Bile Glucose Agar CM0485 or on to lactose-containing media for confirmation of LF or NLF status. Further tests must be made to confirm the identity of the isolate.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared medium at 2-8°C.