產(chǎn)品名稱:MLCB瓊脂 MLCB Agar
供應(yīng)商:Oxoid
貨號:CM0783
規(guī)格:500g
產(chǎn)品說明：
Directions
Suspend 49.0g in 1 litre of distilled water. Mix and bring gently to the boil with frequent agitation to dissolve the medium completely. Cool to 50°C and pour approximately 20ml into sterile Petri dishes
Technique
Dry the surface of the agar before use.
Inoculate the medium heavily with the specimen or enrichment culture and incubate for 18-24 hours at 35°C.
Examine for typical large purple-black colonies of H2S positive salmonella. Search carefully for H2S negative strains that atypically grow as large mauve-grey colonies with a cratered centre. A proportion may show a black `bulls eye’.
Pick all colonies presumed to be Salmonella spp. and confirm by biochemical and serological testing.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates of medium at 2-8°C.