產(chǎn)地:oxoid 貨號:CM0854B 操作說明: Sheep Blood Agar Base was developed to meet the demand for an especially nutritious blood agar base which would permit the maximum recovery of organisms without interfering with their haemolytic reactions when used with sheep blood. Sheep Blood Agar Base is based on the formulation of Blood Agar Base No.2 CM0271. Blood Agar Base No.2 when supplemented with sheep blood was occasionally found to result in a mixed haemolytic reaction (alpha and beta haemolysis) for some Group A Streptococci (Streptococcus pyogenes). These mixed haemolytic reactions were due to trace amounts of fermentable carbohydrates in yeast extract and the physiological differences of sheep blood when compared to horse blood1. Having identified the causes of the mixed haemolytic reactions, the Sheep Blood Agar Base was formulated to be compatible with sheep blood. Comparisons with other blood agar bases supplemented with sheep blood have shown that with Sheep Blood Agar Base the growth of many bacteria - especially the fastidious streptococci - is considerably improved, and the expected beta haemolytic reaction is achieved with Streptococcus pyogenes. Directions Suspend 40g in 1 litre of distilled water and bring to the boil to dissolve completely. Sterilise by autoclaving at 121°C for 15 minutes. Cool to 50°C and aseptically add 7% sterile sheep blood. 保存條件: Store the dehydrated medium at 10-30°C and use before the expiry date on the label. Store the prepared medium at 2-8°C. 綿羊血瓊脂基礎(chǔ) 500g 可以配制12.5 升培養(yǎng)基CM0854B Tryptose Blood Agar Base 營養(yǎng)極佳的培養(yǎng)基,所配的血平板培養(yǎng)苛養(yǎng)菌 |