產(chǎn)品名稱:酵母霉菌瓊脂 Yeast And Mould Agar
供應(yīng)商:Oxoid
貨號(hào):CM0920
規(guī)格:500g
產(chǎn)品說明：
Directions
Suspend 41.0g in 1 litre of distilled water and bring to the boil to dissolve. Sterilise by autoclaving at 121°C for 15 minutes. The medium may be rendered selective after sterilisation by acidifying to pH 4.0 with 12-15ml of Lactic Acid SR0021 after cooling to 50°C. Do not reheat after making this addition. Mix well and pour into sterile Petri dishes.
Technique
Cultures on plates or membrane filters are incubated for 3 days at 25°C under strictly anaerobic conditions. Continue incubation of the cultures under aerobic conditions for a further 2 days. Yeast colonies may be very small immediately following anaerobic incubation but will increase in size in air. Mould growth may become completely unrestricted after 3 days in air.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
The prepared medium may be stored for up to 2 weeks at 2-8°C.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel