Caspase-3 Colorimetric Assay Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (400 or 405 nm)
• Sample type- Cell and tissue lysates
• Species reactivity- Mammalian
• Kit size- Convenient sizes (25, 100, 200, 400 assays)
• Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Features & Benefits:
• Simple one-step procedure; takes 1-2 hours
• Fast and convenient
• Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
Kit components:
• Cell Lysis buffer
• 2X Reaction Buffer
• DEVD-pNA (4 mM)
• DTT (1 M)
• Dilution Buffer
Description:
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-3/CPP32 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence DEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in CPP32 activity.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.