FractionPREP™ Cell Fractionation Kit
Product Overview
Kit Summary:
• Sample type- Cell culture (adherent and suspension), fresh and frozen tissues
• Species reactivity- Mammalian
• Applications- 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.
Features & Benefits:
• Simple procedure; takes less than 1 hour
• Fast and convenient
• The kit is designed for serial sample preparation of four distinct protein fractions including cytosol/particulate/cytoskeleton/nuclear fractions, from one sample.
Kit components:
• Cytosol Extraction Buffer (CEB)
• Membrane Extraction Buffer-A (MEB-A)
• Membrane Extraction Buffer-B (MEB-B)
• Nuclear Extraction Buffer (NEB)
• DTT (1 M)
• Protease Inhibitor Cocktail (lyophilized)
Description:
This FractionPREP™ Cell Fractionation system is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.