Bioluminescence Cytotoxicity Assay Kit Product Overview Kit Summary: • Detection method- Luminescence • Sample type- Cell culture (adherent and suspension) • Species reactivity- Mammalian Features & Benefits: • Simple one-step procedure; takes less than 30 minutes • Fast and convenient • The assay is highly sensitive and can be fully automatic for high throughput studies. Kit components: • AK Detection Reagent (Lyophilized) • AK Assay Buffer Description: Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Adenylate kinase (AK) is a ubiquitous protein present in all eukaryotic and prokaryotic cells and rapidly release into culture medium upon damage of the plasma membrane. The Bioluminescence Cytotoxicity Assay Kit is based on the measurement of AK in a simple one-step procedure involving two chemical reactions. The first reaction is the conversion of ADP to ATP by adenylate kinase that was released from the damaged cells. The second reaction utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or beta counter. The assay is highly sensitive and can be fully automatic for high throughput. Storage Conditions: -20°C Shipping Conditions: gel pack USAGE: For Research Use Only! Not For Use in Humans.