NAD/NADH Quantitation Colorimetric Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (450 nm)
• Sample type- Cell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- BioVision’s NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio.
Features & Benefits:
• Simple procedure; takes ~2 hours
• Fast and convenient
• Kit contains the necessary reagents for accurate measurement of NAD and NADH and their ratio.
Kit components:
• NADH/NAD Extraction Buffer
• NAD Cycling Buffer
• NAD Cycling Enzyme Mix
• NADH Developer
• Stop Solution
• NADH Standard (MW:763)
Description:
Assay of nicotinamide nucleotides is of continual interest in the studies of energy transforming and redox state of cells or tissues. BioVision’s NADH/NAD Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADH, NAD and their ratio. The NAD Cycling Enzyme Mix in the kit specifically recognizes NADH/NAD in an enzyme cycling reaction. There is no requirement to purify NADH/NAD from samples. The reaction specifically detects NADH and NAD, but not NADP nor NADPH. The enzyme cycling reaction significantly increases the detection sensitivity and specificity. NADt (NAD and NADH) or NADH can be easily quantified by comparing with standard NADH.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.