Visfatin (human) Elisa Assay Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (450 nm)
• Sample type- Serum
• Species reactivity- Mammalian
• Applications-This human Visfatin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of visfatin. Works well with rat samples.
Features & Benefits:
• Simple procedure
• Fast and convenient
Kit components:
• Antibody coated 96-well plate (12 x 8 well strips, with absorbed monoclonal antibody to human visfatin)
• 10X Wash concentrate, 50 ml
• 5X Diluent, 50 ml (for reagent dilution)
• 1X Secondary antibody, 12 ml (Rabbit Polyclonal antibody against human visfatin)
• 100X Detector, 150 µl (HRP conjugated anti-rabbit IgG)
• Standard, recombinant human visfatin (16 ng), lyophilized
• QC sample= 2 positive control of recombinant human visfatin (3 ng/ml, 0.5 ng/ml)
• Substrate, 12 ml (chromogenic reagent)
• Stop solution, 12 ml (1 M H3PO4)
• Plate sealer (3 sealers)
Description:
Visfatin, an adipocytokine that is highly enriched in the visceral fat of both humans and mice and whose expression level in plasma increases during the development of obesity. Visfatin corresponds to pre-B cell colony-enhancing factor (PBEF), a 52-kD cytokine expressed in lymphocytes. PBEF is an inflammatory cytokine that plays a requisite role in the delayed neutrophil apoptosis of sepsis. Visfatin exerted insulin-mimetic effects in cultured cells and lowered plasma glucose levels in mice. It was found that visfatin binds to and activates the insulin receptor. Plasma level of visfatin in patients with type 2 diabetes mellitus was elevated, suggesting that measurement of plasma visfatin provides a relevant tool for understanding metabolic diseases.
Storage Conditions:
+4°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.