CETP Inhibitor Drug Screening Kit (Fluorometric)
Product Overview
Kit Summary:
• Detection method- Fluorescence (Ex/Em 465/535 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
Features & Benefits:
• Simple procedure; takes ~60 minutes
• Fast and convenient
• Supplied with 100 positive control assays and all other necessary reagents for screening of CETP inhibitors.
Kit components:
• Donor Molecule
• Acceptor Molecule
• CETP Assay Buffer (10X)
• Rabbit Serum (CETP source)
Description:
Cholesteryl ester transfer protein (CETP) is a member of the lipid transfer/lipopolysaccharide binding protein gene family. CETP transfers neutral lipids from high-density lipoprotein (HDL) to very low-density lipoprotein (VLDL) and is present in normal human plasma and serum. The CETP Drug Screening Kit uses a donor molecule containing a fluorescent self-quenched neutral lipid that is transferred to an acceptor molecule in the presence of CETP (rabbit serum). CETP-mediated transfer of the fluorescent neutral lipid to the acceptor molecule results in an increase in fluorescence (Excitation: 465 nm; Emission: 535 nm). Inhibitor of CETP will inhibit the lipid transfer and therefore decrease fluorescence intensity. The kit provides sufficient reagents for 100 CETP inhibitor screening assays.
Storage Conditions:
RT
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.