PLTP Activity Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method- Fluorescence (Ex/Em 465/535 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
Features & Benefits:
• Simple procedure; takes ~40 minutes
• Fast and convenient
• Supplied with 100 positive control assays and all other necessary reagents for screening of PLTP inhibitors.
Kit components:
• PLTP Donor Molecule
• PLTP Acceptor Molecule
• PLTP Assay Buffer (10X)
• Positive Control (Rabbit Serum)
Description:
Plasma phospholipid transfer protein (PLTP) is thought to play a major role in the facilitated transfer of phospholipids between lipoproteins and in the modulation of high-density lipoprotein (HDL) particle size and composition. PLTP-facilitated lipid transfer activity is related to HDL and LDL metabolism, as well as lipoprotein lipase activity, adiposity, and insulin resistance. The PLTP Activity Assay Kit uses a donor molecule containing a fluorescent self-quenched phospholipid that is transferred to an acceptor molecule in the presence of PLTP. PLTP-mediated transfer of the fluorescent phospholipid to the acceptor molecule results in an increase in fluorescence (Excitation: 465 nm; Emission: 535 nm).
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.