Free Fatty Acid Quantification Colorimetric/Fluorometric Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Application- The kit has a detection limit 2 µM free fatty acid in variety samples.
Features & Benefits:
• Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit contains all necessary reagents for rapid, sensitive and accurate measurement of Adipolysis in cell culture samples
Kit components:
• Fatty Acid Assay Buffer
• Fatty Acid Probe in DMSO
• ACS Reagent
• Enzyme Mix
• Enhancer
• Palmitic Acid Standard (1nmol/µl)
Description:
Fatty Acids play very important roles in normal metabolism and many disease developments. They are precursors to a number of bioactive classes of compounds such as prostaglandins, leucotrienes and others, and have been implicated in diverse functions such as autism, immune system and inflammation response. BioVision’s Free Fatty Acid Quantification Kit provides a convenient, sensitive enzyme-based method for detecting the long-chain free fatty acids in various biological samples, such as serum, plasma and other body fluids, food, growth media, etc. In the assay, Fatty Acids are converted to their CoA derivatives, which are subsequently oxidized with the concomitant generation of color or fluorescence. C-8 (octanoate) and longer fatty acids can then be easily quantified by either colorimetric (spectrophotometry at λ = 570 nm) or fluorometric (at Ex/Em = 535/587 nm) methods with detection limit 2 µM free fatty acid in variety samples.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.