Glucose and Sucrose Colorimetric/Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method-Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, monitoring glucose level during fermentation and glucose feeding in protein expression processes.
• Species reactivity- Mammalian
Features & Benefits:
• Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit provides all necessary buffers and reagents for a accurate assay of glucose and sucrose individually in various biological samples
Kit components:
• Glucose Assay Buffer
• Glucose Probe (Lyophilized)
• Dimethylsulfoxide (DMSO; H₂O-free)
• Invertase (Lyophilized)
• Glucose Enzyme Mix (Lyophilized)
• Sucrose Standard (100 nmol/µl)
Description:
Glucose (C₆H₁₂O₆; FW: 180.16) and sucrose (C₁₂H₂₂O₁₁; FW:342.3) are the important fuel sources to generate universal energy molecule ATP. Measurement of glucose or sucrose level can be very important in both research and development process. Sucrose is a disaccharide which can be converted into one glucose and one fructose when adding Invertase. BioVision’s Glucose and Sucrose Assay Kit provides a convenient means for measuring glucose and sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). To measure glucose level, glucose oxidase specifically oxidizes free-glucose generating a compound that reacts with the glucose probe to produce resorufin, which can be detected colorimetrically (O.D. 570 nm) or fluorometrically (Ex/Em 535/587). To measure sucrose, invertase can be added to the reaction to convert sucrose to free glucose and fructose, so total glucose level can be measured. Then the sucrose level = Total Glucose – Free Glucose.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans.