Description
Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus. The enzyme catalyzes 5'=>3' synthesis of DNA, has no detectable 3'=>5' exonuclease (proofreading) activity and possesses low 5'=>3' exonuclease activity. In addition, Taq DNA Polymerase exhibits deoxynucleotidyl transferase activity, which frequently results in the addition of extra adenines at the 3'-end of PCR products.
Recombinant Taq DNA Polymerase is ideal for standard PCR of templates 5 kb or shorter.
Applications
Routine PCR amplification of DNA fragments up to 5 kb (1).
Generation of PCR product for TA cloning.
DNA labeling (2-4).
DNA sequencing (5).
Quality Control
The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests. Functionally tested in PCR.
Storage Buffer
The enzyme is supplied in:
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween 20 and 50% (v/v) glycerol.
Features
Thermostable – half life is more than 40 min at 95°C.
Generates PCR products with 3’-dA overhangs.
Supplied with two buffers – 10X Taq Buffer with KCl and 10X Taq Buffer with (NH4)2SO4. The latter allows for PCR at wide range of magnesium concentrations (see Fig.1) and decreases unspecific priming.
Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides).