Klenow Fragment Exonuclease Minus
產(chǎn)地：MBI
貨號(hào)：EP0422
Applications
Random-primed DNA labeling (2-4).
Labeling by fill-in 5'-overhangs of dsDNA.
Strand displacement amplification (SDA) (5).
DNA sequencing by the Sanger method (6).
Description
Klenow Fragment, exo-, is the Large Fragment of DNA Polymerase I. It exhibits 5'=>3' polymerase activity, but lacks the 3'=>5' and 5'=>3' exonuclease activities of DNA Polymerase I. The 3'=>5' exonuclease activity of the enzyme is eliminated by mutations in the 3'=>5'-exonuclease active site (1).