DreamTaq Green PCR Master Mix (2X)現(xiàn)貨進(jìn)口
應(yīng)用：
Routine PCR amplification of DNA fragments up to 6 kb from genomic DNA and up to 20 kb from viral DNA.
RT-PCR.
Genotyping.
Generation of PCR products for TA cloning.
特性：
Convenient – DreamTaq™ Green DNA Polymerase in a ready-to-use mix.
Save time – go directly from PCR to gel electrophoresis.
High yields of PCR products with minimal optimization.
Higher sensitivity compared to conventional Taq DNA polymerase.
Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA.
Robust amplification of difficult templates.
說明：
DreamTaq™ Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq™ DNA Polymerase, optimized DreamTaq™ Green buffer, MgCl2 and dNTPs. The master mix is supplemented with two tracking dyes and a density reagent that allows for direct loading of PCR products on a gel. The dyes in the master mix do not interfere with PCR performance and are compatible with downstream applications such as automated fluorescent DNA sequencing, ligation and restriction digestion.
The master mix retains all features of DreamTaq™ DNA Polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA.
For applications that require PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless DreamTaq™ PCR Master Mix (2X).
成分:
DreamTaq™ DNA Polymerase is supplied in 2X DreamTaq™ Green buffer, dATP, dCTP, dGTP and dTTP 0.4 mM each and 4 mM MgCl2.
DreamTaq™ Green buffer is a proprietary formulation optimized for robust performance in PCR. In addition, it contains a density reagent and two dyes for monitoring electrophoresis progress: the blue dye migrates with 3-5 kb DNA fragments in a 1% agarose gel and the yellow dye migrates faster than 10 bp DNA fragments in 1% agarose gel. The dyes have absorption peaks at 424 nm and 615 nm.