Description
GeneRuler 50 bp DNA Ladder, ready-to-use, is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose or polyacrylamide gels. The ladder is a mixture of chromatography-purified individual DNA fragments.
GeneRuler 50 bp DNA Ladder, ready-to-use, is premixed with 6X DNA Loading Dye for direct loading on gel.
The ladder is supplied with 6X DNA Loading Dye.
Features
Ideal for both DNA sizing and approximate quantification.
Sharp bands.
Bright reference bands (given in red).
Can be directly loaded and are stable at room temperature for 6 months.
Supplied with loading dye for sample DNA.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The absence of nucleases is confirmed by a direct nuclease activity assay.
GeneRuler™ Storage and Loading Buffer (for ready-to-use ladders)
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 0.005% xylene cyanol FF and 10% glycerol.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.
Storage
Store at room temperature or at 4°C for up to 6 months.
For longer periods, store at -20°C.