Description
MassRuler High Range DNA Ladder is specially designed for accurate quantification and sizing of DNA fragments by agarose gel electrophoresis. The intensity of the fragment in each ladder is calibrated against a standard that guarantees the precise quantity of each band. The ladders are mixtures of chromatography-purified, individual DNA fragments.
Features
Accurate DNA sizing and quantification – DNA ladder fragments are quantified spectrophotometrically.
Sharp bands.
Easy-to-remember fragment sizes and quantities.
Ready-to-use – premixed with 6X MassRuler DNA Loading Dye for direct loading and room temperature storage.
Supplied with loading dye for sample DNA.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage and Loading Buffer
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 10% glycerol.
6X MassRuler DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 60% glycerol and 60 mM EDTA.
Storage
Store at room temperature or at 4°C for up to 6 months.
For longer periods, store at -20°C.