Description
Lambda DNA/Eco47I (AvaII) Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Fermentas PureExtreme® restriction enzyme(s), purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.
Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, it can be labeled radioactively or non-radioactively with Klenow Fragment or Klenow Fragment, exo- with the fill-in reaction, see protocols.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The DNA concentration is determined spectrophotometrically.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Features
Sizing and approximate quantification of large DNA fragments.
Sharp bands.
Supplied with loading dye for sample DNA.
Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) and 1 mM EDTA.
6X DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.