Description
NoLimits™ is a collection of 36 highly-purified individual DNA fragments ranging from 10-20,000 bp.
NoLimits™ DNA fragments are not produced by PCR like custom standards from other vendors. Fermentas DNA fragments are produced using specifically designed plasmids purified by patented technology. Plasmid DNA is digested with PureExtreme® restriction enzymes and the individual DNA fragments are chromatography-purified from the digestion mixture. As a result, we offer exceptionally pure DNA fragments that are free of any truncated or degraded molecules. Our custom DNA fragments are the best choice for applications ranging from electrophoresis (both gel and capillary) to HPLC and beyond.
Features
Chromatography-purified individual DNA fragments.
Broad range: 10-20,000 bp.
Sharp peaks during capillary electrophoresis, sharp bands during gel electrophoresis.
Precise DNA concentration.
Applications
Gel electrophoresis.
Capillary electrophoresis.
DNA quantification.
HPLC analysis.
Applications where a custom DNA size standard is required.
Quality Control
Tested on an Agilent 2100 bioanalyzer and by appropriate gel electrophoresis. DNA concentration determined spectrophotometrically. The absence of nucleases confirmed by appropriate tests.
Storage Buffer (TE buffer)
10 mM Tris-HCl (pH 7.6) with 1 mM EDTA.