PageRuler Plus Prestained Protein Ladder
Broad range: 10-250 kDa.
Bright orange reference bands: ~70 kDa and ~27 kDa.
Ready-to-use - supplied in a loading buffer for direct loading on gels.
Sharp bands.
Description
PageRuler™ Prestained Protein Ladders (PageRuler™ Plus Prestained Protein Ladder and PageRuler™ Prestained Protein Ladder) are designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency and approximate sizing of proteins (1-3).
The PageRuler™ Plus Prestained Protein Ladder contains two orange reference bands for easy orientation. It is a mixture of 9 recombinant, highly purified, colored proteins with apparent molecular weights of 10 to 250 kDa. A blue chromophore is coupled to these proteins, and proteins of two reference bands of ~70 kDa and of ~27 kDa are colored with an orange dye.
PageRuler™ Prestained Protein Ladders can be used in Western blotting on PVDF (see protocol for Western Blotting, nylon and nitrocellulose membranes.
Applications
Monitoring of protein migration during SDS- polyacrylamide gel electrophoresis.
Monitoring of protein transfer onto membranes during Western blotting (see protocol for Western Blotting).
Sizing of proteins on SDS-polyacrylamide gels and Western blots.
Composition
0.1-0.2 mg/ml of each protein in 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol.
Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.
Storage
Store at -20°C.
Stable at +4°C for up to 3 months