Description
RiboRuler Low Range RNA Ladder is a mixture of 7 chromatography purified single-stranded RNA transcripts (100-1000 b long), produced from specific templates that contain a fragment of the pTZ19R polylinker and Lambda phage fragments.
Ladder is available in 1 mM EDTA, pH 6.0.
RiboRuler Low Range RNA Ladder is composed of chromatography-purified transcripts what makes them free from NTPs and RNA degradation products. The concentration of each RNA transcript is determined spectrophotometrically (see photo for precise quantities). To minimize the possibility of contamination during the use the ladder is aliquotted in smaller volumes.
RiboRuler Low Range RNA Ladder is optimal for end-labeling using T4 Polynucleotide Kinase, see protocol, and is ideal for Northern blots.
Features
Sharp bands of uniform intensity.
Easy-to-remember band sizes and quantities.
Predetermined concentration of ladder bands allows for approximate RNA quantification.
Supplied with 2X RNA Loading Dye.
Stable for 6 months at -20°C.
Applications
RNA sizing and quantification on native or denaturing gels.
Northern blotting.
Quality Control
Tested in gel electrophoresis. RNA concentration is determined spectrophotometrically. The absence of ribonucleases confirmed by appropriate tests.
Storage
Store at -20°C for 6 months or at -70°C for longer periods.
注：本產(chǎn)品僅用于生命科學(xué)研究實(shí)驗(yàn)，不作為任何藥物及其他用途。