Description
Spectra™ Multicolor Low Range Protein Ladder is designed specifically for small protein analysis. It is a mixture of 6 recombinant, highly purified proteins and synthetic peptides with apparent molecular weights of 1.7 to 40 kDa. The proteins are individually prestained using three different dyes.
The Spectra™ Multicolor Low Range Protein Ladder is ready-to-use: no heating, further dilution or addition of a reducing agent is required before use.
Features
Sharp and stable colors.
Multicolor – easy to track on gel.
Ready to load – no need to boil.
Excellent Western transfer.
Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western Blotting.
Applications
Monitoring of protein migration during SDS-PAGE (1).
Verifying Western transfer efficiency (2-4).
Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots.
Locating a protein of interest for excision from an unstained preparative gel.
Storage
Stable at 4°C for up to 3 months. For long term storage, store at -20°C.
Storage Buffer
62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% (w/v) SDS, 10 mM DTT, 1.5 mM NaN3 and 33% (v/v) glycerol.
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