Caspase-13 Substrate LEED-AFC
Product Overview
Product Name: Caspase-13 Substrate LEED-AFC
Alternate Name/Synonyms: Ac-LEED-AFC, Caspase-13 Substrate, Fluorogenic
Description: Ready-to-use fluorometric substrate for caspase-13 and related caspases that recognize the amino acid sequence LEED. Caspase-13 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEED-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.
Peptide Sequence: Ac-Leu-Glu-Glu-Asp-AFC
Appearance: Liquid
Formulation: DMSO (4 mM)
CAS Number: N/A
Molecular Formula: C₃₂H₃₈F₃N₅O₁₃
Molecular Weight: 757.66
Purity: >98% by HPLC
Solubility:N/A
Storage Temp.: -20°C
Shipping Conditions: gel pack
Handling: Protect from light and moisture
SMILES: CC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(CCC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C
InChi: InChI=1S/C32H38F3N5O13/c1-14(2)10-21(36-15(3)41)31(52)39-19(6-8-24(42)43)28(49)38-20(7-9-25(44)45)29(50)40-22(13-26(46)47)30(51)37-16-4-5-17-18(32(33,34)35)12-27(48)53-23(17)11-16/h4-5,11-12,14,19-22H,6-10,13H2,1-3H3,(H,36,41)(H,37,51)(H,38,49)(H,39,52)(H,40,50)(H,42,43)(H,44,45)(H,46,47)
InChi Key: ZPNCQMJLTSFJQH-UHFFFAOYSA-N
PubChem CID: 44135106
MDL Number: MFCD03452816
USAGE: For Research Use Only! Not For Use in Humans.