Fas/Apo1 Antibody
Product Overview
Antibody Target: Fas/Apo-1
Alternative Name: ALPS1A , APO-1, APT1, CD95 , FAS1, FAIM2, FASTM, LFG, NMP35
Host: Rabbit
Antibody Type: Polyclonal
Isotype: Rabbit IgG
Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 32 of mouse Fas
Accession#: P25446
Gene ID: 14102
Appearance: Colorless liquid
Concentration: 0.5 mg/ml
Formulation: 100 µg (0.5 mg/ml) affinity purified polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Purification: Affinity purified
Species Reactivity: human, mouse, rat
Application: Western blot & Immunohistochemistry
Application & Usage: Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The purified antibody detects a 55 kDa of glycosylated Fas, a 35 kDa native form of Fas and a 29 kDa of cleaved Fas fragment on SDS-PAGE immunoblots.
Storage Temp.: -20 °C
Shipping: gel pack
Background Descriptions: Fas/Apo-1/CD95 is a cell surface type-I membrane glycoprotein with an apparent molecular weight of 44 kDa on SDS-PAGE. Fas is a member of the TNFR family that includes TNFR1, TNFR2, CD27, CD30, and CD40. Binding of Fas-ligand (FasL) to Fas or crosslinking of Fas by anti-Fas monoclonal antibodies leads to apoptosis in Fas expressing cells. Both Fas and TNF-R contain the 70 amino acids cytoplasmic “death domain” that is responsible for transmitting signal for apoptosis.
Handling: The antibody solution should be gently mixed before use.
Usage: For Research Use Only! Not to be used in humans.