Ham's F-12 Nutrient Mixture 
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. Life Technologies offers a variety of Gibco® F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Ham's F-12 is modified as follows:
With
• L-glutamine
• Phenol Red
The complete formulation is available.
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.
Form: Liquid 
Volume: 500 ml 
Glutamine: L-Glutamine 
HEPES Buffer: No HEPES
Product Size: 10 x 500 mL
Phenol Red Indicator: Phenol Red
Sodium Pyruvate Additive: Sodium Pyruvate 
Green Features: Sustainable packaging
Storage conditions: 2°C to 8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture