pBAD-DEST49 Gateway® Destination Vector
The pBAD-DEST49 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli. The pBAD-DEST49 vector features:
• The araBAD promoter for tightly regulated expression in E. coli
• HP-thioredoxin fusion for improved protein yield and solubility
• Enterokinase cleavage site for efficient cleavage of the N-terminal fusion with EKMax™
• C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins
• R sites for efficient recombination with any attL-flanked Gateway® entry vector
Vector Type: pDEST,
pBAD 
Cloning Method: Gateway® 
Promoter: araBAD 
Protein Tag or Fusion: V5 Epitope Tag,
Thioredoxin,
His Tag (6x) 
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site 
Product Size: 6 µg 
Inducing Agent: Arabinose 
Selection Agent (Eukaryotic): None
Constitutive or Inducible System: Inducible 
Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures.