RPMI 1640 Medium, Powder
Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco® RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI is modified as follows:
With Without
• L-glutamine • HEPES
• Phenol Red • Sodium Bicarbonate
The complete formulation is available.
Gibco® RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B12
Glutamine: L-Glutamine 
Phenol Red Indicator: Phenol Red 
Form: Powder 
HEPES Buffer: No HEPES 
Serum Supplementation: Standard Serum Supplementation 
Sodium Pyruvate Additive: No Sodium Pyruvate 
Product Size: 50 L 
Shipping Condition: Room Temperature
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture