Opti-MEM® I Reduced Serum Medium 
Opti-MEM® Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM® is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM® can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. Life Technologies offers a variety of Gibco® Opti-MEM® modifications for a range of cell culture applications.
This Opti-MEM® is modified as follows:
With
• L-glutamine
• Phenol Red
The complete formulation is confidential. For more information, please contact Technical Services.
Gibco® Opti-MEM® is a unique media that contains
Glutamine: L-Glutamine 
Phenol Red Indicator: Phenol Red 
Form: Liquid 
Volume: 500 ml 
Product Size: 10 x 500 mL 
Green Features: Sustainable packaging
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture