Contents
BrdU Labeling Reagent, 1,000x conc., sterile
Washing Buffer concentrate, 10x
Incubation Buffer
Anti-BrdU, (contains nucleases for DNA denaturation)
Anti-mouse-Ig-fluorescein
Background Information
Cell proliferation may be studied by monitoring the incorporation of a radioisotope, [3H]-thymidine, into cellular DNA, followed by autoradiography.
Alternatively, 5-bromo-2'-deoxy-uridine (BrdU) may be used instead of thymidine. Cells that have incorporated BrdU into DNA are easily detected using a monoclonal antibody against BrdU and an enzyme- or fluorochrome-conjugated second antibody
Product Description
Sample material: Cell culture: adherent cells, suspension cells, organ, or explant cultures. Tissue sections (after in vivo labeling with BrdU).
Specificity: Anti-BrdU monoclonal antibody specifically binds to 5-bromo-2'-deoxy-uridine, and shows crossreactivity with 5-iodo-2'-deoxy-uridine (10%). Anti-BrdU shows no crossreactivity with 5'-fluoro-2'-deoxy-uridine or any endogenous cellular component, such as thymidine or uridine.
Benefits
Safe: No radioisotopes are used
Easy to perform: Follows a standard immunofluorescence protocol
Sensitive: Denaturation of DNA with nucleases allows for highly sensitive detection of BrdU
Flexible: Allows double-labeling protocols
Application
The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures, or by in vivo labeling, in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation.
5-Bromo-2'-deoxy-uridine Labeling and Detection Kit I
11296736001 1 kit (for up to 100 tests)