Application
Fast, sensitive and specific colorimetric assay for the quantitative determination of neutral α-glucosidase in life science research applications. The measurement is performed exclusively in human semen samples as a parameter for the evaluation of male infertility.
Contents
Reaction Buffer
Substrate Solution containing 4-nitrophenyl-α-D-glucopyranoside
Stopping Buffer
Castanospermine
Standard Solution containing 4-nitrophenol
Principle
The sample is incubated at neutral pH in the presence of SDS assuring that only neutral α-glucosidase from epididymis is measured. α-Glucosidase cleaves 4-nitrophenol from the substrate 4-nitrophenyl-α-D-glucopyranoside yielding a yellow color which can be measued in cuvettes or microplates.
Note: Contains castanospermine (α-glucosidase inhibitor) for the preparation of the blank.
Quality
The assay is linear between 2 mU/ml and 45 mU/ml. Intra-assay variances are only 1 - 3%; inter-assay variances are up to 5%.
α-Glucosidase Assay
11742027001 1 kit for 30 tests and 10 blanks

名稱	產(chǎn)地	貨號	規(guī)格	價格
α-Glucosidase Assay	ROCHE	11742027001	30t	4724