Application
For the detection of fluorescein-labeled compounds.
ELISA
Immunohisto/cytochemistry
In situ hybridization
Western blot
Product Description
Clone: B13-DE1
IgG class: mouse IgG1
Specificity: The monoclonal antibody (clone B13-DE1) reacts with free and bound fluorescein.
Working concentration:
ELISA: 2 - 4 µg/ml
Immunohisto/cytochemistry: 0.5 - 2 µg/ml
In situ hybridization: 0.2 - 0.4 µg/ml
Western blot: 0.5 - 2 µg/ml
Background Information
To obtain monoclonal antibodies, Balb/c mice were immunized with KLH-bound fluorescein and the spleen cells were subsequently fused with myeloma cells of the cell line NS-1. The antibodies were purified by gel filtration, diluted in 10 M potassium phosphate buffer, 75 mM NaCl, 2% raffinose (w/v), 0.01% 2-methylisothiazolone (MIT) (w/v), pH 7.4, and subsequently lyophilized.
Contents
Lyophilizate, stabilized
Anti-Fluorescein from mouse IgG1 (clone B13-DE1) 
For the detection of fluorescein-labeled compounds.
11426320001 100 µg

名稱	產(chǎn)地	貨號(hào)	規(guī)格	價(jià)格
Anti-Fluorescein from mouse IgG1 (clone B13-DE1)	ROCHE	11426320001	100ug	3199