Collagenase P from Clostridium histolyticum
Application
Collagenase from C. histolyticum is used for the dissociation of tissues for the establishment of primary cell cultures.
Collagenase P is well suited for the isolation of pancreatic islets from mouse and rat (function-tested).
Product Description
EC 3.4.24.3
Enzyme activity:
Collagenase activity: >1.5 U/mg (according to Wünsch) (25°C, 4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as the substrate)
Contaminating enzyme activities: trypsin, clostripain, and total proteolytic activity
Collagenase P has a very high collagenase activity (>1.5 U/mg), and is function-tested for the isolation of mouse pancreatic islets.
Inhibitors:
Collagenase inhibitors: EDTA, EGTA, Cys, His, DTT, 2-mercaptoethanol
Collagenase is not inhibited by serum.
Clostripain inhibitors: TLCK
Trypsin inhibitors: aprotinin, trypsin inhibitor (egg white, soybean)
Specificity: Collagenase degrades native collagen. Clostripain, trypsin-like enzymes, and neutral proteases degrade other proteins as well.
Working concentration: 0.5 - 2.5 mg/ml
Contents
Lyophilizate, non-sterile