Application
Used for the inhibition of proteolytic activity in large volumes in which EDTA may interfere with protein stability or subsequent assays or purification methods ( e.g. , Immobilized Metal Chelate Affinity Chromatography [IMAC]).
Benefits
Easy-to-use: Simply drop a quick-dissolving tablet into your buffer.
Complete protection: Instantly protect your proteins against a broad range of proteases.
Flexible: Performs in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
Safe: Non-toxic inhibitors without risk to you or those around you.
Suitable for IMAC: For the purification of Poly-His-tagged proteins.
Product Description
Starting concentration: 1 tablet contains protease inhibitors sufficient for a 50-ml cell extract.
Specificity: Mixture of several protease inhibitors. For the inhibition of serine, cysteine, but not metalloproteases.
Solubility/Stability: Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1 to 2 weeks at +2 to +8°C or at least 12 weeks at -15 to -25°C. Can be used in thiol-containing solutions at room temperature.
Note: Inhibition efficiency is quickly and sensitively tested with the Universal Protease Substrate (Cat. Nos. 11 080 733 001 or 11 734 334 001).
Quality
Performance-tested with pancreas extract.
05056489001 3 x 20 tablets in glass vials. Each tablet is sufficient for a volume of 50 ml solution
cOmplete, EDTA-free Protease Inhibitor Cocktail Tablets provided in glass vials