cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Tablets provided in a glass vial/蛋白酶抑制劑
Application
Used for the inhibition of proteolytic activity in small volumes in which EDTA may interfere with protein stability or subsequent assays or purification methods (e.g. , Immobilized Metal Chelate Affinity Chromatography [IMAC]).
Benefits
•Easy-to-use: Simply drop a quick-dissolving tablet into your buffer.
•Complete protection: Instantly protect your proteins against a broad range of proteases.
•Flexible: Performs in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
•Safe: Non-toxic inhibitors without risk to you or those around you.
•Suitable for IMAC: For the purification of Poly-His-tagged proteins.
Product Description
Starting concentration: 1 tablet contains protease inhibitors sufficient for a 10-ml cell extract.
Specificity: Mixture of several protease inhibitors. For the inhibition of serine, cysteine, but not metalloproteases.
Solubility/Stability: Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1 to 2 weeks at +2 to +8°C, or at least 12 weeks at -15 to -25°C. Can be used in thiol-containing solutions at room temperature.
Note: Inhibition efficiency is quickly and sensitively tested with the Universal Protease Substrate (Cat. Nos. 11 080 733 001 or 11 734 334 001).
Quality
Performance-tested with pancreas extract.