FuGENE 6 Transfection Reagent  
Non-liposomal formulation for the transfection of eukaryotic cells. New X-tremeGENE DNA Transfection Reagents available from Roche. Order your free sample at www.x-tremegene.roche.com
Application
FuGENE®  6 Transfection Reagent is a non-liposomal multicomponent reagent that is perfect for experiments involving cellular analysis. Its characteristics (listed under "Benefits" below) make it ideal for transfection of a wide range of cells.
Note: To see a list of more than 700 types of cells that have been successfully transfected with FuGENE® 6 Transfection Reagent, including many "hard-to-transfect" cell types, visit our Transfection Special Interest Site and view our Transfection Reference Database. This site also contains much more information about the use of this reagent.
For life science research only.
Product Description
Composition: contains a proprietary blend of lipids and other components in 80% ethanol.
Sterility: filter-sterilized.
Background Information
Mentioned in over 8,500 publications, FuGENE® 6 Transfection Reagent is one of the most widely used transfection reagents in the world.
Contents
Lipid blend in 80% ethanol, sterile-filtered, packaged in glass vials.
Quality
Functional analysis:
Cos 7 cells are transfected in 96 well plates with a reporter gene vector DNA, using 0.3 µl FuGENE® 6 and 0.1 µg DNA or 0.25 µl FuGENE® 6 and 0.1 µg DNA. Reporter gene activity is monitored via a colorimetric test against a reference lot. Activity must be ≥ 75% compared to a reference lot in at least one of the tested amounts, listed above.
Cytotoxicity analysis:
Cell growth is assessed using the Cell Proliferation Reagent WST-1 assay, which provides an indirect measure of cytotoxicity. The remaining cells from the functional analysis are tested for viability.