特價(jià):480元 另備 SIGMA原裝蛋白酶K  貨號(hào):P6556 規(guī)格:100MG 價(jià)格:1221元

Proteinase K, Tritirachium album
Serine protease that exhibits strong proteolytic activity on a wide variety of denatured and native proteins of high molecular weight. Because of its rapid proteolytic inactivation of endogenous nucleases, it can be used for isolation of mRNA and high molecular weight DNA. Preferentially cleaves bonds next to the carbonyl group of N-substituted hydrophobic, aliphatic, and aromatic amino acids. Inhibited by DFP, Hg2+, and PMSF. Not inactivated by metal chelators, sulfhydryl reagents, TLCK, or TPCK.
Product information 
Form  White lyophilized solid 
Formulation  Lyophilized from calcium acetate 
Molar mass  28500 
Specific activity  ≥40 mAnsonU/mg protein 
Unit definition  One unit is defined as the amount of enzyme that will liberate 1.0 µmol tyrosine from casein per min at 37°C, pH 7.5. 
Activity  ≥30 mAnsonU/mg dry weight 
Contaminants  DNase: no detectable nicking activity with pBR322, incubation for 6 h at 37°C; RNase: no ribonuclease activity with MS2 RNA detected after incubation for 6 h at 37°C 
pH value  optimum 7.5 - 12.0 
Solubility  50 mM Tris-HCl, 2 mM calcium acetate, pH 8.0 
EC number  3.4.21.14 
CAS number  39450-01-6 
Store and ship information 
Storage  +2°C to +8°C