First Strand cDNA Synthesis Kit Reliable preparation of templates for RT-PCR The First Strand cDNA Synthesis Kit is designed for the preparation of high quality first strand cDNA from cellular RNA templates. The kit contains MMLV Reverse Transcriptase for superior yields of full-length cDNA. Both oligo(dT) and random hexamer primers are included for a choice of general priming strategies and as alternatives to user-supplied specific primers. A small volume (1-2 µl) of the first strand cDNA reaction can be used in PCR amplification with KOD Hot Start or KOD XL DNA Polymerase. Use this kit in conjunction with the Straight A’s™ mRNA Isolation System and appropriate PCR reagents to amplify rare coding regions Avoid freeze/thaw 是的 Components • 4000 U MMLV Reverse Transcriptase • 200 µl 5X First Strand Buffer • 100 µl 100 mM DTT • 50 µl 10 mM dNTP Mix • 20 µg Oligo(dT) Primer • 10 µg Random Hexamer Primers • 1.5 ml Nuclease-free Water • 1 µg Positive Control RNA • 10 µl Positive Control Primers, 3' AS • 10 µl Positive Control Primers, 5' S 商店和運(yùn)輸信息 Storage Multiple storage requirements Ship Blue Ice Only Multiple Toxicity Values, refer to MSDS