Overnight Express™ Autoinduction NMR Medium - Optimization
Overnight Express™ Autoinduction NMR Medium-Optimization provides reagents to make 1 L of
chemically defined autoinducing liquid culture medium used to optimize cell growth and protein expression before preparing isotopically labeled proteins for NMR spectroscopy. The autoinduction method is based on the differential metabolism of carbon sources in the medium to promote growth to high cell density and to automatically induce protein expression under control of lac promoters, eliminating the need to monitor cell densities or add inducer (IPTG). The system includes four concentrated sterile solutions: OnEx NMR Solution 1 is a blend of carbon sources optimized for tightly regulated uninduced growth to high density, followed by induction; OnEx Solution 2 is a buffer and nitrogen blend that mediates metabolic acid production and provides additional nitrogen for increased protein synthesis; OnEx Solution 3 provides magnesium for maximal cell density; OnEx Solution 4 provides trace metals to minimize growth limitations associated with mineral deficiencies and to satisfy the requirements of metal-containing target proteins, even at high expression levels. The kit also includes unlabeled solid ammonium chloride and reagents to make a non-inducing starter medium, for staged growth without expression and for stable storage of clones. Addition of the four liquid components and a sterile ammonium chloride solution to sterile water results in a chemically- defined medium promoting high cell densities and autoinduction of expression. When the user provides and substitutes 15N-labeled ammonium chloride for the supplied unlabeled reagent, this kit can be used to prepare 15N-labeled proteins.
The Overnight Express Autoinduction System is covered by US Patents 7,560,264, 7,704,722, and 7,759,109. This Product is sold for research use only. Any commercial use of the Product, including the manufacture of proteins for use in the screening of compound libraries, requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
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Storage  Multiple storage requirements
Ship  Multiple Storage Conditions
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