Phleomycin
Phleomycin is a glycopeptide antibiotic of the bleomycin family, isolated from a mutant strain of Streptomyces verticillus. It binds and intercalates DNA thus destroying the integrity of the double helix. Phleomycin is active against most bacteria, filamentous fungi, yeast, plant and animal cells.
Use of phleomycin is recommended for cells poorly sensitive to Zeocin™, i.e. filamentous fungi and some yeasts. Phleomycin resistance is conferred by the Sh ble gene from Streptoalloteichus hindustanus which encodes a protein that binds to phleomycin, inhibiting its DNA cleavage activity.
Typically, phleomycin is used at a concentration of 10 µg/ml for yeasts and 25-150 µg/ml for filamentous fungi.
Working concentration:
- 10 µg/ml for yeasts
- 25-150 µg/ml for filamentous fungi
CAS number: 11006-33-0
Purity: >90%
Molecular weight: 1525
Formula: C55H85O21N20S2Cu-HCl