M-TriDAP
MurNAc-L-Ala-γ-D-Glu-mDAP (M-TriDAP), also called DAP-containing muramyl tripeptide is a peptidoglycan (PGN) degradation product found mostly in Gram-negative bacteria. M-TriDAP is recognized by the intracellular sensor NOD1
(CARD4) and to a lesser extend NOD2 (CARD15). Recognition of M-TriDAP by NOD1/NOD2 induces a signaling
cascade involving the serine/threonine RIP2 (RICK, CARDIAK) kinase which interacts with IKK leading to the activation of NF-κB and the production of inflammatory cytokines such as TNFα and IL-6 [1]. M-TriDAP induces the activation of NF-κB at similar levels to Tri-DAP [2].
M-TriDAP provided by InvivoGen is chemically synthesized and tested using HEK-Blue™ NOD1 and HEK-Blue™ NOD2 Cells.
Note: M-TriDAP is a mixture of MurNAc-L-Ala-γ-D-Glu-D- mDAP and MurNAc-L-Ala-γ-D-Glu-L-mDAP
Specificity: NOD1/NOD2 agonist
Working Concentration: 1 - 10 µg/ml
Endotoxin level:  <0.125 EU/mg
Solubility: 10 mg/ml in water
Molecular weight: 665.64
Synonym: N-acetyl-muramyl-L-Ala-γ-D-Glu-meso- diaminopimelic acid