原裝正品|GenMute siRNA Transfection Reagent for Jurkat Cell
Description:
GenMute™ siRNA Transfection Reagent for Jurkat Cell is pre-optimized for transfecting siRNA to Jurkat cells. Jurkat cells are an immortalized line of T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science because of their ability to produce interleukin 2. Their primary use, however, is to determine the mechanism of differential susceptibility of cancers to drugs and radiation.
Summary of Optimal Transfection Conditions:
Cell culture medium     DMEM with 4.5 g/L glucose, 10% FBS
Confluency of cell on the day of transfection  ~50%
Optimal siRNA concentration    30 nM
Diluent for siRNA and GenMute™ Reagent   1xGenMute™ Transfection Buffer
Incubation Time to Form GenMute™/siRNA Complex  ~15 minutes at RT
Maximal Silencing Efficiency     48 hours
Transfection Results:
Reporter Gene   Renilla Luciferase 
Silencing Efficiency (% ) 80%
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.