原裝正品|GenMute siRNA Transfection Reagent for C2C12 Cell
功能:
GenMute™ siRNA Transfection Reagent for C2C12 Cell is pre-optimized for transfecting siRNA to C2C12 cells. C2C12 cells were originally obtained by Yaffe and Saxel (1977) through selective serial passage of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush injury. These cells were shown to be capable of differentiation. C2C12 cells are a useful model to study the differentiation of non-muscle cells to skeletal muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins and the androhen receptor (AR).
最佳轉(zhuǎn)染條件:
Cell culture medium     DMEM with 4.5 g/L glucose, 10% FBS
Confluency of cell on the day of transfection  ~50%
Optimal siRNA concentration    40 nM
Diluent for siRNA and GenMute? Reagent   1xGenMute? Transfection Buffer
Incubation Time to Form GenMute?/siRNA Complex  ~15 minutes at RT
Maximal Silencing Efficiency     48 hours
Transfection Results:
Reporter Gene   Renilla Luciferase 
Silencing Efficiency (% ) 80%
儲(chǔ)存條件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.