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GenJet In Vitro DNA Tranfection Reagent for PC-12 Cell

產(chǎn)品編號: SL100489-PC12     查看說明書
產(chǎn)品名稱: GenJet In Vitro DNA Tranfection Reagent for PC-12 Cell  .0   訂購此產(chǎn)品 
供應商: SignaGen
規(guī)格: 1.0 ml
目錄價: 1560
庫存狀態(tài): 詢盤
CAS編號:
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產(chǎn)品名稱:GenJet In Vitro DNA Tranfection Reagent for PC-12 Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for PC12 cell is pre-optimized for most efficiently transfecting PC12 cells. PC12 is a cell line derived from a pheochromocytoma of the rat adrenal medulla.  PC12 cells stop dividing and terminally differentiate when treated with nerve growth factor.  This makes PC12 cells useful as a model system for neuronal differentiation.
最佳轉(zhuǎn)染條件:
Confluency of cell on the day of shaving 80%
GenJet™ (µl) : DNA (µg) Ratio   3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency     48 hours
Transfection Results:
Reporter Gene   EGFP
Plasmid    pEGFP-N3 (CMV promoter)
Efficiency (GFP %)  50%
儲存條件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.

 

保存條件: 4°C
說明書地址: 點擊查看詳細
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