產(chǎn)品名稱(chēng):GenJet (Ver. II) DNA In Vitro Transfection Reagent for D 407 Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for D 407 is pre-optimized for transfecting D 407, retinal pigment epithelial cells. Refer to the following optimal transfection conditions for maximal transfection efficiency on D 407 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 50 to 100 transfections in 6 well plates.
最佳轉(zhuǎn)染條件:
Confluency of cell on the day of shaving 80%
GenJet™ (µl) : DNA (µg) Ratio   3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency     48 hours
Transfection Results:
Reporter Gene   EGFP
Plasmid    pEGFP-N3 (CMV promoter)
Efficiency (GFP %)  70%
儲(chǔ)存條件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.