產(chǎn)品名稱:GenJet (Ver. II) DNA In Vitro Transfection Reagent for SHEP Cells
功能:
GenJet™ DNA In Vitro Tranfection Reagent for SHEP is pre-optimized for transfecting SHEP cells. This human neuroblastoma cell line was cloned from a single primary tumor. Unlike other neuroblastoma cell, SHEP cells does not produce IGF-II, expresses five-fold fewer IGF-IRs than SH-SY5Y and dies in serum-free media or following exposure to metabolic stressors.
最佳轉(zhuǎn)染條件:
Confluency of cell on the day of shaving 85%
GenJet™ (µl) : DNA (µg) Ratio   3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency     48 hours
Transfection Results:
Reporter Gene   EGFP
Plasmid    pEGFP-N3 (CMV promoter)
Efficiency (GFP %)  80%
儲存條件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.