產(chǎn)品名稱:GenJet (Ver. II) DNA In Vitro Transfection Reagent for NMuMG Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for NMuMG cells is pre-optimized for transfecting NMuMG cells. NMuMG is mouse mammary gland, normal epithelial. NMuMG cells express different integral surface proteoglycans (e.g. syndecan). They are used for studies of cell adhesion and growth factor-binding molecules as well as for analysis of growth factor effects on cell proliferation.
最佳轉(zhuǎn)染條件:
Confluency of cell on the day of shaving 90%
GenJet™ (µl) : DNA (µg) Ratio   3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet™/DNA Complex 15 minutes at RT
Maximal Efficiency     48 hours
Transfection Results:
Reporter Gene   EGFP
Plasmid    pEGFP-N3 (CMV promoter)
Efficiency (GFP %)  75%
儲(chǔ)存條件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.