產(chǎn)品名稱:GenJet (Ver. II) DNA In Vitro Transfection Reagent for C6 Cell
功能:
GenJet™ DNA In Vitro Tranfection Reagent for C6 Glioma cell is pre-optimized for transfecting C6 glioma cells. The C6 glioma cell line is a widely used cell line in neurobiological research. Compared to primary neural cells, the C6 cell line offers some major advantages like easy accessibility and culture as well as a good availability of high cell numbers. Nevertheless, the biggest disadvantage of the C6 cells is that they are difficult-to-transfect with commonly used non-viral transfection methods. The pre-optimized transfection reagent simplifies the transfection of C6 cells. Transfection efficiencies of over 60% can be easily achieved with excellent cell viabilities of above 90%.
最佳轉(zhuǎn)染條件:
Confluency of cell on the day of shaving 80%
GenJet? (μl) : DNA (μg) Ratio   3:1
Diluent for DNA and Transfection Reagent Serum-free DMEM with 4.5 g/L glucose
Incubation Time to Form GenJet?/DNA Complex 15 minutes at RT
Maximal Efficiency     48 hours
Transfection Results:
Reporter Gene   EGFP
Plasmid    pEGFP-N3 (CMV promoter)
Efficiency (GFP %)  60%
儲(chǔ)存條件:
Store at 4 °C. If stored properly, the product is stable for 12 months or longer.