Lenti-X Tet-On 3G Inducible Expression System (EF1alpha, ZsGreen1) This page describes a two-vector lentivirus format for inducible expression. Click this link to learn more about Clontech's all-in-one Lenti-X Tet-One Inducible Expression Systems.
The Lenti-X Tet-On 3G Inducible Expression Systems combine the broad cellular tropism of lentiviral gene transfer technology with the power and versatility of our state-of-the-art tetracycline-regulated gene expression system. The lentiviral Tet-On-3G system consists of two lentiviral vectors: a regulator vector that stably expresses the Tet-On 3G transactivator protein, and a response vector that contains the TRE3G promoter that controls the expression of your transgene. Also included are a control response vector for luciferase expression, and our premium Lenti-X HTX Lentiviral Packaging System.
How it Works
Doxycycline-controlled “transactivators” are fusion proteins that contain a TetR-derived DNA binding domain joined to three minimal transcription activation domains from VP16 (Tet-On 3G). With very high specificity, the transactivator recognizes and binds to a Tetracycline Response Element (TRE) that is located in the PTRE3G promoter of pLVX-TRE3G, into which you clone your gene of interest. Binding of the transactivator to the TRE promoter results in strong activation of the downstream gene. Tet-On 3G is the premium 3rd generation version of the technology, with the tightest TRE promoter and most sensitive transactivator.
In Tet-On systems, gene expression is activated by the addition of Dox to the growth medium, while in Tet-Off systems, transcription is activated by its withdrawal.
High Titer Lentiviral Packaging
Clontech’s Lenti-X High Titer Packaging System, supplied with each system, generates vastly superior titers than most commercially available packaging systems. You can often transduce an entire dish of target cells with just 10–100 µl of unconcentrated viral supernatant. For more details, visit the Lenti-X HTX Packaging System product page.