Anti-digoxigenin AP-conjungate|Anti-Digoxigenin-AP, Fab fragments from sheep

產(chǎn)品編號(hào)：11093274910 

產(chǎn)品名稱：Anti-digoxigenin AP-conjungate|Anti-Digoxigenin-AP, Fab fragments from sheep  .0    

供應(yīng)商：Roche

規(guī)格：150 U (200 μl)

目錄價(jià)：2875

庫存狀態(tài)：大量現(xiàn)貨

相關(guān)信息：

Anti-Digoxigenin-AP, Fab fragments from sheep

Application

For the detection of digoxigenin-labeled compounds.

•Dot Blot

•ELISA

•Immunohisto/cytochemistry

•In situ hybridization

•Northern blot

•Southern blot

•Western blot

Product Description

Specificity: Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., testosterone).

Working concentration:

Dot blot: 150 mU/ml

ELISA: 150 - 300 mU/ml

Immunohisto/cytochemistry: 250 - 500 mU/ml

In situ hybridization: 1.5 - 7.5 U/ml

Southern blot: 150 mU/ml

Western blot: 250 - 500 mU/ml 

Background Information

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer. 

Contents

Solution, stabilized